Lineas de investigacion

Bacterial biotechnology (BIOBACT)



The research in bacterial biotechnology contributes, by the use of functional genomics and biotechnological processes, to generate quality knowledge useful for the identification and the development of bacterial strains, enzymes, and metabolites to improve the food elaboration processes or the microbial functionality in hosting organisms.

Research Lines

  • Identification, characterization, and genetic improvement of bacteria present in food substrates
  • Identification, characterization, production, and improvement of enzymes with technological interest
  • Development of biochemical and molecular methods for the detection of relevant bacterial traits
  • Systems Biology studies aimed at improving the functionality of bacteria in food processes and host-microbial interactions.


  • Biochemical and molecular techniques for the detection of bacteria and genes with technological interest.
  • Production and purification of recombinant enzymes
  • Genetic expression analysis


  • Procedimiento bi-enzimático de síntesis eficiente de oligosacáridos fructosilados derivados de lactosacarosa, productos obtenidos y su uso en la mejora de la salud gastrointestinal. P201430784. No licenciada. 2014. Francisco Javier Moreno, Marina Diez, Miguel Herrero, Nieves Corzo, Rosario Muñoz, Blanca de las Rivas y María Luisa Jimeno.
  • Biological degradation of ochratoxin A in ochratoxin alpha. PCT/WO2012/025657A1. Non licenced. 2010. Rosario Muñoz Moreno, Blanca de las Rivas González del Rey, Héctor Rodríguez López, Inés Reverón Poján, Emilia García-Moruno, Francesca Doria and Antonella Costantini.
  • Use of lactic bacteria for preserving the flavanols of a food product and preventing the browing of wine. PCT/WO201151494A1 Non-licenced. 2010. Félix López de Felipe Toledano, Rosario Muñoz Moreno and José Antonio Curiel Gámiz.


Group Leader:



  • 91 258 75 70


Lactic acid bacteria, Detection methods, Phenolic compounds, Biogenic amines, Esterases


Reverón, I., Jiménez, N., Curiel, J.A., Peñas, E., López de Felipe, F., de las Rivas, B., Muñoz, R.
“Differential gene expression by Lactobacillus plantarum WCFS1 in response to phenolic compounds reveals new genes involved in tannin degradation”
Appl. Environ. Microbiol. (2017) 83:e03387-16 (DOI:10.1128/AEM.03387-16)

Reverón, I., Plaza-Vinuesa, L., Franch, M., de las Rivas, B., Muñoz, R., López de Felipe, F.
“Transcriptome-based analysis in Lactobacillus plantarum WCFS1 reveals new insights into resveratrol effects at system level”
Mol. Nutr.Food Res. (2018) 62:1700992 (DOI:10.1002/mnfr.201700992)

Santamaría, L., Reverón, I., López de Felipe, F., de las Rivas, B., Muñoz, R.
“Ethylphenols formation by Lactobacillus plantarum: Identification of the enzyme involved in vinylphenol reduction”
Appl. Environ. Microbiol. (2018) 84:e01064-18 (DOI:10.1128/AEM.01064-18)

Esteban-Torres, M., Santamaría L, Cabrera-Rubio, R., Plaza-Vinuesa L, Crispie, F., de las Rivas, B., Cotter, P., Muñoz, R.
“A diverse range of human gut bacteria have the potential to metabolize the dietary component gallic acid”
Appl. Environ. Microbiol. (2018) 84:e01558-18 (DOI:10.1128/AEM.01558-18)

Plaza-Vinuesa, L., Hernández-Hernández, O., Moreno, F. J., de las Rivas, B., Muñoz, R.
“Unraveilling the diversity of glycoside hydrolase family 13 α-amylases from Lactobacillus plantarum WCFS1”
Microb Cell Fact. (2019) 18:183 (DOI:10.1186/s12934-019-1237-3)

Reverón, I., Plaza-Vinuesa, L., Santamaría, L., Oliveros, J. C., de las Rivas, B., Muñoz, R, López de Felipe, F.
“Transcriptomic evidence of molecular mechanisms underlying the response of Lactobacillus plantarum WCFS1 to hydroxytyrosol”
Antioxidants (2020) 9:442 (doi:10.3390/antiox9050442)

Projects, Contracts, Agreements

FEDER projects

Lactobacillus plantarum as a model for the study of the interaction with phenolic compounds in food fermentation and in adaptation to the gastrointestinal tract

Main Researcher: R. Muñoz / Start Date: 01/01/2015 / End Date: 12/31/2018 / Institution: MINECO/ERDF, EU / Reference number: AGL2014-52911-R
Research Group: Bacterial biotechnology (BIOBACT)

Carbohydrate-active enzymes from Lactobacillus plantarum WCFS1: genetic, functional and structural characterization

Main Researcher: B. de las Rivas and R. Muñoz / Start Date: 01/01/2018 / End Date: 09/30/2022 / Institution: AEI/ERDF, EU / Reference number: AGL2017-84614-C2-2-R
Research Group: Bacterial biotechnology (BIOBACT)

PhD Thesis, Master's Thesis and Degree Thesis

Display PhD Thesis, Master's Thesis and Degree Thesis

Tesis Doctoral: “Identification and characterization of glycosyl hydrolases in Lactobacillus plantarum WCFS1” (2020)

Author: Laura Plaza Vinuesa / Director: Rosario Muñoz, Blanca de las Rivas y José Miguel Mancheño

Institution: Universidad Complutense de Madrid

Tesis Doctoral: “Identification of reductases of hydroxycinnamic acids and its derivatives in Lactobacillus plantarum WCFS1” (2017)

Author: Laura Santamaría Rubio / Director: Rosario Muñoz, Blanca de las Rivas y Félix López de Felipe

Institution: Universidad Complutense de Madrid