Lineas de investigacion

Functional food of vegetable origin and health (ALIVEFSA)



Study of the health effects of different vegetable food components such as indigestible carbohydrates (dietary fibre and oligosaccharides), among other prebiotics.

Research Lines

  • Nutritional evaluation of prebiotics (oligosaccharides and fibre) as a source of functional ingredients
  • High hydrostatic pressure (HHP) treatment to optimize nutrient bioavailability.
  • Value enhancement of vegetable byproducts from the food industry.
  • Edible seaweeds as a source of biologically active compounds.


  • Development of national funded research projects
  • Development of patents for the utilization of vegetable byproducts from agrofood industry
  • Optimization of methods and techniques for the development and nutritional evaluation of new functional food ingredients
  • Contracts with industry, scientific and technological support or technology transfer to agrofood industries
  • Training of scientific researchers in Nutrition and health


  • Método para la obtención de oligosacáridos vegetales. Nº de solicitud: P200930375. No licenciada. 2009. Pérez-Cózar M.L., Redondo-Cuenca A, Villanueva-Suárez M.J. y Rupérez P.
  • Producto alimentario ligero de configuración esférica hueca a base de gluten inflado por vapor de agua y procedimiento de obtención/ Light, hollow and spherical food product made of inflated gluten and method of preparation. Secreto Industrial nº 4.754 de 2019 Titularidad: 100% CSIC Fecha prioridad: 29-12-2019, registro ante Notario Inventores: Dres. Antonio Diego Molina García y Pilar Rupérez Antón (ICTAN-CSIC) Licenciado a la empresa: TERWICK DIELLOR 2021 S.L. Fecha contrato de licencia: 14-8-2020.


Group Leader:

  • Pilar Rupérez


  • Pilar Rupérez (Investigador Científico)
  • Ana Mª Veses Alcobendas – (Technician)
  • Elena Pérez López – (Predoctoral fellow)


  • +34 915492300


Prebiotics, Indigestible carbohydrates, Oligosaccharides, Dietary fibre, Vegetable byproducts, Soybean okara


  • Pérez-López E, Veses AM, Redondo N, Tenorio-Sanz MD, Villanueva MJ, Redondo-Cuenca A, Marcos A, Nova E, Mateos-Aparicio I, Rupérez P. Soybean Okara modulates gut microbiota in rats fed a high-fat diet. Bioactive Carbohydrates & Dietary Fibre 16, 100–107 (2018) DOI:10.1016/j.bcdf.2018.09.002 2.
  • Pérez-López E, Mateos-Aparicio I, Rupérez P. High hydrostatic pressure aided by food-grade enzymes as a novel approach for Okara valorization. Innov Food Sci Emerg Technol 42, 197-203 (2017) DOI:10.1016/j.ifset.2017.07.012 3.
  • Arshadi M, Mateos-Aparicio I, Rupérez P, et al. Pre-treatment and extraction techniques for recovery of added value compounds from wastes throughout the agro-food chain. Green Chem 18, 6160-6204 (2016) DOI: 10.1039/c6gc01389a 4.
  • Pérez-López E, Cela D, Costabile A, Mateos-Aparicio I, Rupérez P. In vitro fermentability and prebiotic potential of soyabean Okara by human faecal microbiota. Br J Nutr 116, 1116-1124 (2016) DOI:10.1017/S0007114516002816 5.
  • Pérez-López E, Mateos-Aparicio I, Rupérez P. Okara treated with high hydrostatic pressure assisted by Ultraflo® L: Effect on solubility of dietary fibre. Innov Food Sci Emerg Technol 33, 32-37 (2016) DOI:10.1016/j.ifset.2015.12.017 6.
  • Pérez-López E, Mateos-Aparicio I, Rupérez P. Low molecular weight carbohydrates released from Okara by enzymatic treatment under high hydrostatic pressure. Innov Food Sci Emerg Technol 38, 76-82 (2016) DOI:10.1016/j.ifset.2016.09.014

Projects, Contracts, Agreements

FEDER projects

Improvement of the prebiotic functionality in agrofood byproducts by simultaneous treatment with hydrolytic enzymes and high hydrostatic

Main Researcher: M. P. Rúperez and A. D. Molina / Start Date: 12/30/2016 / End Date: 12/31/2020 / Institution: AEI/ERDF, EU / Reference number: AGL2016-77056-R
Research Group: Group of Biophysical Approaches to Food, Food Processing and Preservation (FoodCRYOPHYS)

PhD Thesis, Master's Thesis and Degree Thesis

Display PhD Thesis, Master's Thesis and Degree Thesis

Tesis Doctoral: Enhancement of prebiotic functionality of artichoke by-products (9-10-2017)

Author: Elisa Giambra / Director: Araceli Redondo y Pilar Rupérez

Institution: Università Degli Studi di Milano, Facoltà di Scienze Agrarie e Alimentari, Milán, Italia

Tesis Doctoral: Functionality improvement of soybean okara by simultaneous treatment with high hydrostatic pressure aided by food-grade enzymes (2017)

Author: Elena Pérez López / Director: Pilar Rupérez e Inmaculada Mateos-Aparicio

Institution: Universidad Complutense de Madrid, Facultad de Farmacia