Metabolism and bioactivity of phytochemicals (BIOCELL)

MEMBERS

Group Leader: Laura Bravo and Luis Goya

Members:

  • Laura Bravo (Research Professor)
  • Luis Goya (Research Investigator)
  • Sonia Ramos (Tenured Scientist)
  • Raquel Mateos (Tenured Scientist)
  • M. Angeles Martín (Senior Technician)
  • Beatriz Sarriá (Senior Technician)
  • Shenli Wang (Predoctoral fellowl)
  • Milagros Sánchez-Prieto Borja (Senior Technician)

Contact: +34  – 91 544 5607 – lbravoarrobaictan.csic.es  y  luisgoyaarrobaictan.csic.es

DESCRIPTION

Objectives:

The team of Metabolism and bioactivity of phytochemical carries out basic and applied research to study the health benefits of bioactive compounds proceeding mainly from plant-derived foodstuff.

Research Lines:

  • Antioxidant capacity tested in vitro, ex vivo (cell model of oxidative stress) and in vivo.
  • Bioavailability and metabolism of phytochemicals.
  • Molecular mechanisms of action and cell signalling pathways.
  • Nutritional human studies in healthy voluntaries and risk groups. Preclinic studies in animals.

Skills:

  • Analysis of bioactive compounds (polyphenols, methylxanthines, dietary fiber, prebiotics) in foodstuff and biological samples.
  • Evaluation of in vitro antioxidant capacity (FRAP, ABTS, ORAC, etc.), ex vivo (oxidative stress cell model) and in vivo. Biomarkers
  • Cell bioaccessibility (transport, metabolism) of phytochemicals, tissue distribution in animals and pharmacokinetic bioavailability studies in humans. Analysis of metabolites (LC-DAD, LC-MS, LC-QTOF).
  • Transformed cell lines and animal models of dyslipemia, diabetes and cancer for biochemical and molecular mechanisms of action studies using phytochemicals to combat the pathologies.
  • Human intervention studies. Validation of the health effects of functional food (healthy and at-health risk populations, CVR, etc)

Keywords: Bioactive compounds, Polyphenols & phytochem, Oxidative stress, Bioavailability, Cell signalling pathways, Intervention studies

SCI PUBLICATIONS

  • Cordero-Herrera I, Martín MA, Fernández-Millán E, Alvarez C, Goya L, Ramos S. Cocoa flavanols improve hepatic lipid metabolism in Zucker Diabetic Fatty rats and human hepatic cells. Role of PKC. J Funct Foods 17, 761-773 (2015). DOI: 10.1016/j.jff.2015.06.033.
  • Cordero-Herrera I, Martín MA, Escrivá F, Alvarez C, Goya L, Ramos S. Cocoa-rich diet attenuates hepatic insulin resistance in ZDF rats by modulating glucose homeostasis and insulin signaling. J Nutr Biochem 26, 702-712 (2015). DOI: 10.1016/j.jnutbio.2015.01.009.
  • Martín MA, Ramos S, Mateos R, Marais J, Bravo-Clemente L, Khoo K, Goya L. Chemical characterization and chemo-protective activity of cranberry phenolic powders in a model cell culture. Response of the antioxidant defences and regulation of signaling pathways. Food Res Int 71, 68-82 (2015). DOI: 10.1016/j.foodres.2015.02.022.
  • Cordero-Herrera I, Martín MA, Goya L, Ramos S. Cocoa flavonoids protect hepatic cells against high glucose-induced oxidative stress. Relevance of MAPKs. Mol Nutr Food Res 59, 597-609 (2015). DOI: 10.1002/mnfr.201400492.
  • Fernandez-Millan E, Cordero-Herrera I, Ramos S, Escrivá F, Alvarez C, Goya L, Martín MA. Cocoa-rich diet attenuates beta cell mass loss and function in young Zucker Diabetic Fatty rats by preventing oxidative stress and beta cell apoptosis. Mol Nutr Food Res 59, 820-824 (2015). DOI: 10.1002/mnfr.201400746.
  • Cordero-Herrera I, Martín MA, Goya L, Ramos, S. Cocoa intake ameliorates hepatic oxidative stress in young Zucker Diabetic Fatty rats. Food Res Int 69, 194-201 (2015). DOI: 10.1016/j.foodres.2014.12.039.
  • Mateos R, Madrona A, Pereira-Caro G, Dominguez V, Cert RM, Parrado J, Sarriá B, Bravo L, Espartero JL. Synthesis and antioxidant evaluation of isochroman-derivatives of hydroxytyrosol: structure-activity relationship. Food Chem 173, 313-20 (2015).  DOI: 10.1016/j.foodchem.2014.10.036.
  • Sarriá B, Martínez-López S, Sierra-Cinos JL, Garcia-Diz L, Mateos R, Bravo L. Effects of bioactive constituents in functional cocoa products on cardiovascular health in humans. Food Chem. 174, 214–218 (2015). DOI: 10.1016/j.foodchem.2014.11.004.
  • Blanch M, Rosales R, Mateos R, Perez-Gago MB, Sanchez-Ballesta MT, Escribano MI, Merodio C. Effects of high CO2 levels on fermentation, peroxidation, and cellular water stress in Fragaria vesca stored at low temperature in conditions of unlimited O2. Revista: J. Agric. Food Chem. 63:761-768 (2015). DOI: 10.1021/jf505715s.
  • Ahmed Mobasher M, de Toro-Martín J, González-Rodríguez A, Ramos S, Letzig LG, James LP, Muntané J, Álvarez C, Valverde AM. Essential role of Protein Tyrosine Phosphatase 1B in the modulation of insulin signaling by acetaminophen in hepatocytes. J Bio Chem 289, 29406-29419 (2014). DOI: 10.1074/jbc.M113.539189.
  • Martín MA, Cordero-Herrera I, Bravo L, Ramos S, Goya L. Cocoa flavanols show beneficial effects in cultured pancreatic beta cells and liver cells to prevent the onset of type 2 diabetes. Food Res Int 63, 400-408 (2014). DOI: 10.1016/j.foodres.2014.05.006.
  • Fernández-Tomé S, Ramos S, Cordero-Herrera I, Recio I, Goya L,  Hernández-Ledesma B. In vitro chemoprotective effect of bioactive peptide lunasin against oxidative stress in human HepG2 cells. Food Res Int 62, 793-800 (2014). DOI: 10.1016/j.foodres.2014.04.054.
  • Martín MA, Fernandez-Millan E, Ramos S, Bravo L, Goya L. Cocoa flavonoid epicatechin protects pancreatic beta cell viability and function against oxidative stress. Mol Nutr Food Res 58, 447-456 (2014). DOI: 10.1002/mnfr.201300291.
  • Fernandez-Millan E, Ramos S, Alvarez C, Bravo L, Goya L, Martín MA. Microbial phenolic metabolites improve glucose-stimulated insulin secretion and protect pancreatic beta cells against tert-butyl hydroperoxide-induced toxicity via ERKs and PKC pathways. Food Chem Toxicol 66, 245-253 (2014). DOI: 10.1016/j.fct.2014.01.044.
  • Bravo L, Mateos R, Sarriá B, Lecumberri E, Ramos S, Goya L. Hypocholesterolaemic and antioxidant effects of yerba mate (Ilex paraguariensis) in high-cholesterol fed rats. Fitoterapia 92, 219-229 (2014). DOI: 10.1016/j.fitote.2013.11.007.
  • Cordero-Herrera I, Martín MA, Goya L, Ramos S. Cocoa flavonoids attenuate high glucose-induced insulin signalling blockade and modulate glucose uptake and production in human HepG2 hepatic cells. Food Chem Toxicol 64, 10-19 (2014). DOI: 10.1016/j.fct.2013.11.014
  • Sarriá B, Martínez-López S, Sierra-Cinos JL, Garcia-Diz L, Mateos R, Bravo  L. Regular consumption of a cocoa product improves the cardiometabolic profile in healthy and moderately hypercholesterolemic adults. Br J Nutr.111(1), 122-134 (2014).
    DOI: 10.1017/S000711451300202X.
  • Sáyago-Ayerdi SG, Mateos R, Ortíz-Basurto RI, Largo C, Serrano J, Granado-Serrano AB, Sarriá B, Bravo L, Tabernero M. Effects of consuming diets containing Agave tequilana dietary fibre and jamaica calyces on body weight gain and redox status in hypercholesterolemic rats. Food Chem 14, 54-59 (2014). DOI: 10.1016/j.foodchem.2013.10.004.
  • Martínez-López S, Sarriá B, Sierra-Cinos JL, Goya L, Mateos R, Bravo L. Realistic intake of a flavanol-rich soluble cocoa product increases HDL-cholesterol without inducing anthropometric changes in healthy and moderately hypercholesterolemic subjects. Food Funct 5(2),364-374 (2014). DOI: 10.1039/c3fo60352k.
  • Tabernero M, Sarriá B, Largo C, Martínez-López S, Madrona A, Espartero JL, Bravo L, Mateos R. Comparative evaluation of the metabolic effects of hydroxytyrosol and its lipophilic derivatives (hydroxytyrosyl acetate and ethyl hydroxytyrosyl ether) in hypercholesterolemic rats.  Food Funct 5(7),1556-1563 (2014). DOI: 10.1039/c3fo60677e-
  • Martínez-López S, Sarriá B, Permanyer J, Mateos R, Bravo L. Theobromine, caffeine, and theophylline metabolites in human plasma and urine after consumption of soluble cocoa products with different methylxanthine contents. Food Res Inter. 63, 446-455 (2014). DOI: 10.1016/j.foodres.2014.03.009.
  • Baeza G, Amigo-Benavent M, Goya L, Sarriá B, Mateos R, Bravo L. Green coffee hydroxycinnamic acids but not caffeine protect Hep-G2 cells against oxidative stress. Food Res Inter 62, 1038-1046 (2014). DOI: 10.1016/j.foodres.2014.05.035
  • Martínez-López S, Sarriá B, Baeza G, Mateos R, Bravo L. Pharmacokinetics of caffeine and its metabolites in plasma and urine after consuming a soluble green/roasted coffee blend by healthy subjects. Food Res Inter. 64, 125-133 (2014).
    DOI: 10.1016/j.foodres.2014.05.043.
  • Trujillo M, Gallardo E, Madrona A, Bravo L, Sarriá B, González-Correa JA, Mateos R, Espartero JL. Synthesis and antioxidant activity of nitrohydroxytyrosol and its acyl derivatives. J Agric Food Chem 42, 10297-10303 (2014). DOI: 10.1021/jf503543x.
  • Pantaleón-Velasco MR, Ruiz-López II, Pérez-Silva A, Bravo-Clemente L, Mateos-Briz MR, Ruiz-Espinosa H & Vivar-Vera MA. Antioxidant and functional properties of a high dietary fiber poder from carambola (Averrhoa carambola L.) pomace. Int. J. Food Sci. Technol. 49:2101-2110 (2014) . DOI: 10.1111/ijfs.12519.n.
  • Tan S-A, Goya L, Ramanathan S, Sulaiman SF, Alam M., Navaratnam V, Shu-Chien AC. Chemopreventive effects of standardized papaya leaf fraction on oxidatively stressed human liver cells. Food Res. Int. 64:387-395 (2014). DOI: 10.1016/j.foodres.2014.06.040
  • Martínez-López S, Sarriá B, Baeza G, Mateos R, Bravo L. Pharmacokinetics of caffeine and its metabolites in plasma and urine after consuming a soluble green/roasted coffee blend by healthy subjects. Food Res Int 64, 125-133 (2014).
  • Martínez-López S, Sarriá B, Gómez-Juaristi M, Goya L, Mateos R, Bravo L. Theobromine, caffeine, and theophylline metabolites in human plasma and urine after consumption of soluble cocoa products with different methylxanthine contents. Food Res Int 63, 446-455 (2014).
  • Pantaleón-Velasco MR, Ruiz-López II, Pérez-Silva A, Bravo-Clemente L, Mateos-Briz MR, Ruiz-Espinosa H & Vivar-Vera MA. Antioxidant and functional properties of a high dietary fiber poder from carambola (Averrhoa carambola L.) pomace. Int J Food Sci Technol 49, 2101-2110 (2014).
  • Tan S-A, Goya L, Ramanathan S, Sulaiman SF, Alam M, Navaratnam V, Shu-Chien AC. Chemopreventive effects of standardized papaya leaf fraction on oxidatively stressed human liver cells. Food Res Int 64, 387-395 (2014).
  • Tabernero M, Sarriá B, Largo C, Martínez-López S, Madrona A, Espartero JL, Bravo L, Mateos R. Comparative evaluation of the metabolic effects of hydroxytyrosol and its lipophilic derivatives (hydroxytyrosyl acetate and ethyl hydroxytyrosyl ether) in hypercholesterolemic rats. Food Funct 5, 1556-1563 (2014).
  • Sáyago-Ayerdi SG, Mateos R, Ortíz-Basurto RI, Largo C, Serrano J, Granado-Serrano AB, Sarriá B, Bravo L, Tabernero M. Effects of consuming diets containing Agave tequilana dietary fibre and jamaica calyces on body weight gain and redox status in hypercholesterolemic rats. Food Chem 148, 154-159 (2014).
  • Fernandez-Millan E, Ramos S, Alvarez C, Bravo L, Goya L, Martín MA. Microbial phenolic metabolites improve glucose-stimulated insulin secretion and protect pancreatic beta cells against tert-butyl hydroperoxide-induced toxicity via ERKs and PKC pathways. Food Chem Toxicol 66, 245-253 (2014)
  • Cordero-Herrera I, Martín MA, Goya L, Ramos S. Cocoa flavonoids attenuate high glucose-induced insulin signaling blockade and modulate glucose uptake and production in human HepG2 hepatic cells. Food Chem Toxicol 64, 10-19 (2014).
  • Bravo L, Mateos R, Sarriá B, Lecumberri E, Ramos S, Goya L. Hypocholesterolaemic and antioxidant effects of yerba mate (Ilex paraguariensis) in high-cholesterol fed rats. Fitoterapia 92, 219-229 (2014).
  • Martínez-López S, Sarriá B, Sierra-Cinos JL, Goya L, Mateos R, Bravo L. Realistic intake of a flavanol-rich cocoa product increases HDL-cholesterol without inducing anthropometric changes in healthy and moderately hypercholesterolemic subjects. Food Funct 5, 364-374 (2014).
  • Sarriá B, Martínez-López S, Sierra-Cinos JL, Garcia-Diz L, Mateos R, Bravo L. Regular consumption of a cocoa product improves the cardiometabolic profile in healthy and moderately hypercholesterolemic adults. Br J Nutr 111, 122-134 (2014).
  • Martín MA, Fernandez-Millan E, Ramos S, Bravo L, Goya L. Cocoa flavonoid epicatechin protects pancreatic beta cell viability and function against oxidative stress. Mol Nutr Food Res 58, 447-456 (2014)
  • Martín MA, Cordero-Herrera I, Bravo L, Ramos S, Goya L. Cocoa flavanols show beneficial effects in cultured pancreatic beta cells and liver cells to prevent the onset of type 2 diabetes. Food Res Int 63, 400-408 (2014)
  • Baeza G, Amigo-Benavent M, Sarriá B, Goya L, Mateos R, Bravo L. Green coffee hydroxycinnamic acids but not caffeine protect human HepG2 cells against oxidative stress. Food Res Int 62, 1038-46 (2014).
  • Fernández-Tomé S, Ramos S, Cordero-Herrera I, Recio I, Goya L, Hernández-Ledesma B. In vitro chemoprotective effect of bioactive peptide lunasin against oxidative stress in human HepG2 cells. Food Res Int 62, 793-800 (2014).
  • Navarro M, Amigo-Benavent M, Mesias M, Baeza G, Gökmen V, Bravo L, Morales FJ. An aqueous pomegranate seed extract ameliorates oxidative stress of human hepatoma HepG2 cells. J Sci Food Agric 98, 1622-1627 (2014).
  • Amigo-Benavent M, Clemente A, Caira S, Stiuso P, Ferranti P, del Castillo MD. The use of phytochemomics to evaluate the bioavailability and bioactivity of antioxidant peptides of soybean beta-conglycinin. Electrophoresis 35, 1582-1589 (2014).
  • Mateos R, Pereira-Caro G, Bacon JR, Bongaerts R, Sarriá B, Bravo L, Kroon PA. Anticancer activity of olive oil hydroxytyrosyl acetate in human adenocarcinoma Caco-2 Cells. J Agric Food Chem 61, 3264-3269 (2013).
  • Pereira-Caro G, Mateos R, Traka MH, Bacon JR, Bongaerts R, Sarriá B, Bravo L, Kroon PA. Hydroxytyrosyl ethyl ether exhibits stronger intestinal anticarcinogenic potency and effects on transcript profiles compared to hydroxytyrosol. Food Chem 138, 1172-1182 (2013).
  • Cordero-Herrera I, Cuello S, Goya L, Madrid Y, Bravo L, Camara C, Ramos S. Molecular mechanisms of selenocystine protective effects against methylmercury-induced cell damage in human HepG2 cells. Food Chem Toxicol 59, 554-563 (2013).
  • Rodríguez-Ramiro I, Ramos S, López-Oliva ME, Agis-Torres A, Bravo L, Goya L, Martín MA. Cocoa flavonoids prevent inflammation in colon of azoxymethane-treated rats and in TNF-α-stimulated Caco-2 cells. Brit J Nutr 110, 206-215 (2013).
  • Quéguineur B, Goya L, Ramos S, Martín MA, Mateos R, Guiry MD, Bravo L. Effect of phlorotannin-rich extracts of Ascophyllum nodosum and Himanthalia elongata (Phaeophyceae) on cellular oxidative markers in human HepG2 cells. J Appl Phycol 25, 1-11 (2013).
  • Martín MA, Goya L, Ramos S. Potential for preventive effects of cocoa and cocoa polyphenols in cancer. Food Chem Toxicol 56, 336-351 (2013).
  • Mobasher MA, González-Rodriguez A, Santamaría B, Ramos S, Martín MA, Goya L, Rada P, Letzig L, James LP, Cuadrado A, Martín-Pérez J Simpson KJ, Muntané J, Valverde AM. Protein tyrosine phosphatase 1B modulates GSK3β/Nrf2 and IGF-1R signaling pathways in acetaminophen-induced hepatotoxicity. Cell Death Dis 4, e626 (2013).
  • Martín MA, Ramos S, Cordero-Herrera I, Bravo L, Goya L. Cocoa phenolic extract protects pancreatic beta cells against oxidative stress. Nutrients 5, 2955-2968 (2013).
  • Blanch M, Rosales R, Goya L, Sánchez-Ballesta MT, Escribano MI, Merodio C. NADP-malic enzyme and glutathione reductase contribute to glutathione regeneration in Fragaria vesca fruit treated with protective CO2 concentrations. Postharvest Biol Technol 86, 431-436 (2013).
  • Del Castillo MD, Martinez-Saez N, Amigo-Benavent M, Silvan JM. Phytochemomics and other omics for permitting health claims made on foods. Food Res Int 54, 1237-1249 (2013).
  • Amigo-Benavent, M, Nitride, C, Bravo, L, Ferranti, P, del Castillo, MD. Stability and bioactivity of a Bowman-Birk inhibitor in orange juice during processing and storage. Food Funct 4 (7), 1051-1060 (2013). DOI:10.1039/c3fo30354c.
  • Queguineur B, Goya L, Ramos S, Martín MA, Mateos R, Guiry MD, Bravo, L. Effect of phlorotannin-rich extracts of Ascophyllum nodosum and Himanthalia elongata (Phaeophyceae) on cellular oxidative markers in human HepG2 cells. J Appl Phycol 25, 1-11 (2013). DOI 10.1007/s10811-012-9832-2
  • Panzella L, Verotta L, Goya L, Ramos S, Martín MA, Bravo L, Napolitano A, d’Ischia M. Synthesis and bioactivity profile of a family of 5-S-lipoylhydroxytyrosol-based multidefence antioxidants with sizeable (poly)sulphide chain. J Agric Food Chem 61, 1710-1717 (2013). DOI 10.1021/jf302690c
  • Martín MA, Goya L, Ramos R. Potential for preventive effects of cocoa and cocoa polyphenols in cancer. Food Chem Toxicol 56, 336-351 (2013). DOI 10.1016/j.fct.2013.02.020
  • Rodríguez-Ramiro I, Ramos S, López-Oliva E, Agis-Torres A, Bravo L, Goya L, Martín MA Cocoa polyphenols prevent inflammation in the colon of azoxymethane-treated rats and in TNF-α-stimulated Caco-2 cells. Brit J Nutr (2013) DOI: 10.1017/S0007114512004862
  • Cordero-Herrera I, Martín MA, Bravo L, Goya L, Ramos S. Cocoa flavonoids improve insulin signalling and repress glucose production via AKT and AMPK in HepG2 cells. Mol Nutr Food Res 57, 974-985 (2013). DOI 10.1002/mnfr.201200500
  • Pereira-Caro G, Mateos R, Traka MH, Bacon JR, Bongaerts R, Sarriá B, Bravo L, Kroon PA. Hydroxytyrosyl ethyl ether exhibits stronger intestinal anticarcinogenic potency and effects on transcript profiles compared to hydroxytyrosol. Food Chem 138, 1174-1182 (2013) DOI 10.1016/j.foodchem.2012.11.118
  • Mateos R, Pereira-Caro G, Bacon JR, Bongaerts R, Sarriá B, Bravo L, Kroon PA. Anticarcinogenic activity of olive oil hydroxytyrosyl acetate in human adenocarcinoma Caco-2 cells. J Agric Food Chem 61, 3264-3269 (2013). DOI 10.1021/jf305158q
  • Sarriá B, Martínez-López S, Sierra-Cisnos JL, García-Diz L, Mateos R, Bravo L. Regular consumption of a cocoa product improves the cardio-metabolic profile in healthy and moderately hypercholesterolaemic adults. Brit J Nutr (2013) DOI: 10.1017/S000711451300202X
  • Cordero-Herrera I, Martín MA, Bravo L, Goya L, Ramos S. Epicatechin gallate induces cell death through p53 activation via p38 and JNK in human SW480 colon cancer cells. Nutr Cancer 65, 718-728 (2013) DOI: 10.1080/01635581.2013.795981
  • Cordero-Herrera I, Cuello S, Goya L, Madrid Y, Bravo L, Camara C, Ramos S. Molecular mechanisms of selenocystine protective effects against methylmercury-induced cell damage in human HepG2 cells. Food Chem Toxicol (2013). DOI.org/10.1016/j.fct.2013.06.057
  • Sarria B, Martínez-López S, Fernández-Espinosa A, Gómez-Juaristi M, Goya L, Mateos R, Bravo L. Effects of regularly consuming dietary fibre rich cocoa products on bowel habits in healthy subjects: a free-living, two-stage, randomized, crossover, single-blind intervention. Nutr Metab 9, 33 (2012). DOI: 10.1186/1743-7075-9-33.
  • Queguineur B, Goya L, Ramos S, Martín MA, Mateos R, Guiry MD, Bravo L. Phloroglucinol: antioxidant properties and effects on celular oxidative markers in human HepG2 cell line. Food Chem Toxicol 50, 2886-2893 (2012). DOI: 10.1016/j.fct.2012.05.026.
  • Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. Quercetin modulates Nrf2 and glutathione-related defences via p38 in HepG2 cells. Chem Bio Int 195, 154-164 (2012). DOI 10.1016/j.cbi.2011.12.005
  • Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. Quercetin attenuates NFkB pathway against TNF-alpha-induced inflammation in hepatic cells. Nutr Cancer 64, 588-598 (2012). DOI:10.1080/01635581.2012.661513
  • Pereira-Caro G, Sarriá B, Madrona A, Espartero JL, Escuderos ME, Bravo L, Mateos R. Digestive stability of hydroxytyrosol, hydroxytyrosyl acetate and alkyl hydroxytyrosyl ethers. Int J Food Sci Nutr 63, 703-707 (2012). DOI: 10.3109/09637486.2011.652943
  • León-González A, Mateos R, Ramos S, Martín MA, Sarriá B, Martín-Cordero C, López-Lázaro M, Bravo L, Goya L. Chemo-protective activity and characterization of phenolic extracts from Corema album. Food Res Int 49, 728-738 (2012). DOI 10.1016/j.foodres.2012.09.016
  • Rodríguez-Ramiro I, Ramos S, Bravo L, Goya L, Martín MA. Procyanidin B2 induces Nrf2 translocation and glutathion-S-transferase P1 expresión and via ERKs and p38-MAPK pathways and protect human colonic cells against oxidative stress. Eur J Nutr 51, 881-92 (2012). DOI 10.1007/s00394-011-0269-1
  • Sarriá B, Mateos R, Sierra-Cinos J, Goya L, García-Diz L, Bravo L. Hypotensive, hypoglycaemic and antioxidant effects of consuming a cocoa product in moderately hypercholesterolemic humans. Food Funct 3, 867-874 (2012). DOI 10.1039/c2fo10267f
  • Sarriá B, Mateos R, Ramos S, Martín MA, Espartero JL, Bravo L, Goya L. Nitroderivatives of olive oil phenols protect HepG2 cells against oxidative stress. Food Chem Toxicol 50, 3752-8 (2012). DOI 10.1016/j.fct.2012.07.030
  • Sarria B, Martínez-López S, Fernández-Espinosa A, Gómez-Juaristi M, Goya L, Mateos R, Bravo L. Effects of regularly consuming dietary fibre rich cocoa products on bowel habits in healthy subjects: a free-living, two-stage, randomized, crossover, single-blind intervention. Nutr Metab 9, 33 (2012). DOI: 10.1186/1743-7075-9-33.
  • Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. Quercetin modulates Nrf2 and glutathione-related defences via p38 in HepG2 cells. Chem Bio Int 195, 154-164 (2012). DOI: 10.1016/j.cbi.2011.12.005.
  • Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. Quercetin attenuates NFkB pathway against TNF-alpha-induced inflammation in hepatic cells. Nutr Cancer 64, 588-598 (2012). DOI: 10.1080/01635581.2012.661513.
  • Pereira-Caro G, Mateos R, Sarriá B, Cert R, Goya L, Bravo, L. Hydroxytyrosyl acetate contributes to the protective effects against oxidative stress of virgin olive oil. Food Chem 131, 869-878 (2012). DOI: 10.1016/j.foodchem.2011.09.068.
  • Martín MA, Ramos S, Rodríguez-Ramiro I, Mateos R, Bravo L, Goya L. Signal transduction pathways involved in the chemo-preventive effect of dietary antioxidants: study in HepG2 as a cell culture model. Current Nutr Food Sci 8, 112-121 (2012).
  • Rodríguez-Ramiro I, Ramos S, Bravo L, Goya L, Martín MA. Procyanidin B2 and a cocoa polyphenolic extract inhibit acrylamide-induced apoptosis in human Caco-2 cells by preventing oxidative stress and activation of JNK pathway. J Nutr Biochem 22, 1186-1194 (2011). DOI: 10.1016/j.jnutbio.2010.10.005.
  • Ramos S, Rodríguez-Ramiro I, Martín MA, Goya L, Bravo L. Dietary flavanols exert different effects on antioxidant defences and apoptosis in Caco-2 and SW480 colon cancer cells. Toxicol In Vitro 25, 1771-1781 (2011). DOI: 10.1016/j.tiv.2011.09.007.
  • Rodríguez-Ramiro I, Ramos S, López-Oliva ME, Agis-Torres A, Mateos R, Gómez-Juaristi M, Bravo L, Goya L, Martín MA. Cocoa rich diet prevents azoxymethane-induced colonic preneoplastic lesions in rats by restraining oxidative stress and cell proliferation and inducing apoptotic signalling. Mol Nutr Food Res 55, 1895-1899 (2011). DOI.10.1002/mnfr.2011100363.
  • Gómez-Juaristi M, González-Torres L, Bravo-Clemente L, Vaquero P, Bastida S, Sanchez-Muniz FJ. Beneficial effects of chocolate on cardiovascular health. Nutr Hosp 26, 289-292 (2011). DOI: 10.3305/nh.2011.26.2.5016.
  • Pereira-Caro G, Sarriá B, Madrona A, Espartero JL, Goya L, Bravo L, Mateos R. Alkyl hydroxytyrosyl ethers show protective effects against oxidative stress in HepG2 cells. J Agric Food Chem 59, 5964-5976 (2011).
  • Mateos R, Pereira-Caro G, Bravo L, Saha S, Cert A, Redondo-Horcajo M, Kroon PA. Acetylation of hydroxytyrosol enhances its transport across differentiated Caco-2 cell monolayers. Food Chem 125, 865-872 (2011).
  • Madrona A, Pereira-Caro G, Bravo L, Mateos R, Espartero JL. Preparation and antioxidant activity of tyrosyl and homovanillyl ethers. Food Chem 129, 1169-1178 (2011).
  • Rodríguez-Ramiro I, Martín MA, Ramos S, Bravo L, Goya L. Olive oil hydroxytyrosol reduces toxicity evoked by acrylamide in human Caco-2 cells by preventing oxidative stress. Toxicology 288, 43-48 (2011).
  • Rodríguez-Ramiro I, Martín MA, Ramos S, Bravo L, Goya, L. Comparative effects of cocoa flavanols on antioxidant defenses and oxidant-induced stress in Caco2 cells. Eur J Nutr 50, 313-322 (2011).
  • Martín MA, Ramos S, Granado-Serrano AB, Rodríguez-Ramiro I, Trujillo M, Bravo L, Goya L. Hydroxytyrosol induces antioxidant/detoxificant enzymes activity and Nrf2 translocation via ERKs and PI3K/AKT pathways in HepG2 cells. Mol Nutr Food Res 54, 956-966 (2010).
  • Martín MA, Ramos S, Mateos R, Izquierdo-Pulido M, Bravo L, Goya L. Protection of human HepG2 cells against oxidative stress induced by the flavonoid epicatechin. Phytotherapy Res 24, 503-509 (2010).
  • Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. Quercetin modulates NF-κB and AP-1/JNK pathways to induce cell death in human hepatoma cells. Nutr Cancer 62, 390-401(2010).
  • Granado-Serrano AB, Martín MA, Haegeman G, Goya L, Bravo L, Ramos S. Epicatechin induces NF-κB, AP-1 and Nrf2 via PI3K/AKT and ERK signalling in HepG2 cells. Brit J Nutr 103, 168-179 (2010).
  • Martín MA, Granado-Serrano AB, Ramos S, Izquierdo-Pulido M, Bravo L, Goya L. Cocoa flavonoids up-regulate antioxidant enzymes activity via ERK1/2 pathway to protect against oxidative stress-induced apoptosis in HepG2 cells. J Nutr Biochem 21, 196-205 (2010).
  • Pereira-Caro G, Bravo L, Madrona A, Espartero JL, Mateos R. Uptake and metabolism of new synthetic lipophilic derivatives, hydroxytyrosyl ethers, by HepG2 cells. J Agric Food Chem 58, 798-806 (2010).
  • Pereira-Caro G, Mateos R, Saha S, Madrona A, Espartero JL, Bravo L, Kroon PA. Transepithelial transport and metabolism of new lipophilic ether derivatives of hydroxytyrosol by enterocyte-like Caco-2/TC7 cells. J Agric Food Chem 58, 11501-11509 (2010).
  • Cuello S, Goya L, Madrid Y, Campuzano S, Pedrero M, Bravo L, Cámara C, Ramos S. Molecular mechanisms of methylmercury-induced cell death in human hepatoma HepG2 cells. Food Chem Toxicol 48, 1405-1411 (2010).
  • Cofrades S, Lopez-Lopez I, Bravo L, Ruiz-Capillas C, Bastida S, Larrea MT, Jimenez-Colmenero F. Nutritional and antioxidant properties of different brown and red Spanish seaweeds. Food Sci Technol Int 16, 361-370 (2010).
  • Breusing N, Grune T, Andrisic L, Atalay M, Bartosz G, Biasi F, Borovic S, Bravo L, Casals I, Casillas R, Dinischiotu A, Drzewinska J, Faber H, Fauzi NM, Gajewska A, Gambini J, Gradinaru D, Kokkola T, Lojek A, Luczaj W, Margina D, Mascia C, Mateos R, Meinitzer A, Mitjavila MT, Mrakovcic L, Munteanu MC, Podborska M, Poli G, Sicinska P, Skrzydlewska E, Viña J, Wiswedel I, Zarkovic N, Zelzer S, Spickett CM. An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples. Free Radic Res 44, 1203-1215 (2010).
  • Granado-Serrano AB, Martín MA, Goya L, Bravo L, Ramos S. Time course regulation of survival pathways by epicathechin on HepG2. J Nutr Biochem 20, 115-124 (2009).
  • Martín MA, Ramos S, Mateos R, Rufián-Henares JA, Morales FJ, Bravo L, Goya, L. Biscuit melanoidins of different molecular masses protect human HepG2 cells against oxidative stress. J Agric Food Chem 57, 7250-7258 (2009).
  • Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. A diet rich in cocoa attenuates N-nitrosodiethylamine-induced liver injury in rats. Food Chem Toxicol 47, 2499-2506 (2009).
  • Goya L, Martín MA, Ramos S, Mateos R, Bravo L. A cell culture model for the assessment of the chemopreventive potential of antioxidant compounds. Current Nutr Food Sci 5, 56-64 (2009).
  • Gómez-Estaca J, Bravo L, Montero P, Gómez-Guillén C. Antioxidant properties of tuna-skin and bovine-hide gelatine films induced by the addition of oregano and rosemary extracts. Food Chem 112, 18-25 (2009).
  • Lopez-Lopez I, Bastida S, Ruiz-Capillas C, Bravo L, Larrea MT, Sanchez-Muniz FJ, Cofrades S, Jimenez-Colmenero F. Composition of low-salt meat gel/emulsion systems as affected by types of added edible seaweeds. Meat Sci 83, 492-498 (2009).
  • Pereira-Caro G, Madrona A, Bravo L, Espartero JL, Alcudia F, Cert A, Mateos R. Antioxidant activity evaluation of alkyl hydroxytyrosyl ethers, a new class of hydroxytyrosol derivatives. Food Chem 115, 86-91 (2009).
  • Bravo L, Lecumberri E, Mateos R, Ramos S, Izquierdo-Pulido M, Goya L. A diet rich in dietary fibre from cocoa improves lipid profile. AgroFOOD Industry High Tech 19, 22-24 (2008).
  • Martín MA, Ramos S, Mateos R, Granado-Serrano AB, Izquierdo-Pulido M, Bravo L, Goya L. Protection of human HepG2 cells against oxidative stress by cocoa phenolic extract. J Agric Food Chem 56, 7765-7772 (2008).
  • Ramos S, Moulay L, Granado-Serrano AB, Vilanova O, Muguerza B, Goya L, Bravo L. Hypolipidemic effect in colesterol-fed rats of a soluble fibre-rich product obtained from cocoa husks. J Agric Food Chem 56, 6985-6993 (2008).
  • Granado-Serrano AB, Martín MA, Bravo L, Goya L, Ramos S. Time course of quercetin-induced apoptosis in human hepatoma cells involves the differential regulation of cell survival/proliferation pathways. Mol Nutr Food Res 52, 457-464 (2008).
  • Cofrades S, Lopez-Lopez I, Solas MT, Bravo L, Jimenez-Colmenero F. Influence of different types and proportions of added edible seaweeds on characteristics of low-salt gel/emulsion meat systems. Meat Sci 79, 767-776 (2008).
  • Ramos S. Cancer chemoprevention and chemotherapy: dietary polyphenols and signalling pathways. Mol Nutr Food Res 52, 507-526 (2008).
  • Ramos S. Effects of dietary flavonoids on apoptotic pathways related to cancer chemoprevention. J Nutr Biochem 18, 427-442 (2007)
  • Bravo L, Goya L, Lecumberri E. LC/MS characterization of phenolic constituents of mate (Ilex paraguariensis, St. Hil.) and its antioxidant activity compared to commonly consumed beverages. Food Res Int 40, 393-405 (2007).
  • Goya L, Mateos R, Bravo L. Effect of the olive oil phenol hydroxytyrosol on human hepatoma HepG2 cells. Protection against oxidative stress induced by tert-butylhydroperoxide. Eur J Nutr 46, 70-78 (2007).
  • Granado-Serrano AB, Martín MA, Izquierdo-Pulido M, Goya L, Bravo L, Ramos S. 2007. Molecular mechanisms of (-)-epicatechin and chlorogenic acid on the regulation of the apoptotic and survival/proliferation pathways in a human hepatoma cell line (HepG2). J Agric Food Chem 55, 2020-2027 (2007).
  • Lecumberri E, Mateos R, Izquierdo-Pulido M, Rupérez P, Goya L, Bravo L. Dietary fibre composition and antioxidant and physico-chemical properties of a fibre-rich product from cocoa (Theobroma cacao L.). Food Chem 104, 948-954 (2007).
  • Goya L, Delgado-Andrade C, Rufián-Henares JA, Bravo L, Morales FJ. Effect of coffee Melanoidin on human hepatoma HepG2 cells. Protection against oxidative stress induced by tertbutyl hydroperoxide. Mol Nutr Food Res 51, 536-545 (2007).
  • Lecumberri E, Goya L, Mateos R, Alía M, Ramos S, Izquierdo-Pulido M, Bravo L. 2007. A diet rich in dietary fiber from cocoa (Theobroma cacao L.) reduces serum triglycerides, cholesterol and malondialdehyde in hypercholesterolemic rats. Nutrition 23, 332-341 (2007).
  • Cuello S, Ramos S, Mateos R, Martín MA, Madrid Y, Cámara C, Bravo L, Goya L. Effect of Se-methylselenocysteine on human hepatoma HepG2 cells. Protection against oxidative stress induced by tert-butylhydroperoxide. Anal Bioanal Chem 389, 2167-2178 (2007).
  • Mateos R, Bravo L. Chromatographic and electrophoretic methods for the analysis of biomarkers of oxidative damage to macromolecules (DNA, lipids and proteins). J Sep Sci 30, 175-191 (2007).

NON-SCI PUBLICATIONS

  • Martín MA, Ramos S, Rodriguez-Ramiro I, Mateos R, Bravo L, Goya L. Signal Transduction Pathways Involved in the Chemo-Preventive Effect of Dietary Antioxidants: Study in HepG2 as a Cell Culture Model. Current Nutrition and Food Science 8, 112-121 (2012).
  • Tan SA, Ramos S, Martín MA, Mateos R, Harvey M, Ramanathan S, Najimudin N, Alam M, Bravo L, Goya L. Protective effects of papaya extracts on tert-butyl hydroperoxide mediated oxidative injury to human liver cells (An in-vitro study). Free Radicals and Antioxidants 2(3), 10-19 (2012).
  • Mateos R, Pereira-Caro G, Goya L, Bravo L. Biodisponibilidad y metabolismo de compuestos fenólicos del aceite de oliva virgen. Alimentación, Nutrición y Salud, Vol. 18, No 1, 2-9 (2011).
  • Bravo L. Fibra dietética y salud. Compuestos bioactivos asociados a la fibra dietética. Katedra Kellogg’s, Vol I (3), 1-16 (2011).
  • Bravo L, Sarriá B, Gómez-Juaristi M, Martínez-López S, Mateos R. Posibles efectos beneficiosos en salud asociados al consumo de café verde. Alimentación, Nutrición y Salud, 17 (3), 1-9 (2010) .
  • Goya L, Mateos R, Martín MA, Ramos S, Bravo L. Uptake, Metabolism and Biological Effect of the Olive Oil Phenol Hydroxytyrosol in Human HepG2 Cells. En: Olives and Olive Oil in Health and Disease Prevention. Victor R. Preedy and Ronald R. Watson, Eds. Oxford Adademic Press, Elsevier, San Diego, CA, USA, Ch 127, pp 1157-1165. ISBN 978-0-12-374420-3. (2010).
  • Martín MA, Ramos S, Granado-Serrano AB, Rodríguez-Ramiro I, Mateos R, Bravo L, Goya L. Molecular mechanisms involved in Hydroxytyrosol protection against oxidative stress in HepG2 cells. En: Olive Oil and Health. James D. Corrigan, Ed. Nova Science Publishers, Inc. ISBN: 978-1-61728-653-7. (2010)
  • Mateos R, Pereira-Caro G, Goya L, Bravo L. Bioavailability and Metabolism of Virgin Olive Oil Phenolic Compounds”. En: Olive Oil and Health. James D. Corrigan, Ed. Nova Science Publishers, Inc. ISBN: 978-1-61728-653-7. (2010).
  • Granado-Serrano AB, Ramos S. Molecular targets of quercetin in cancer chemoprevention. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, Vol. 5, No 35 (2010). DOI: 10.1079/PAVSNNR20105035.
  • Ramos S. Polyphenols and cancer chemoprevention: modulation of signalling pathways En: New Research on Dietary Polyphenols and Health. Neville Basallo, Ed. Nova Science Publishers Inc. ISBN: 978-1-60456-349-8. (2008).
  • Ramos S. Polyphenols and apoptosis: the case of flavonoids In: New Cell Apoptosis Research. Frank Columbus, Ed. Nova Science Publishers Inc. ISBN: 1-60021-430-4. (2007).
  • Bravo L, Mateos R. Analysis of Flavonoids in Functional Foods and Nutraceuticals. En: Methods of Analysis for Functional Foods and Nutraceuticals. 2nd Edition. W.J. Hurst, Ed. CRC Press, Boca Ratón, FL (USA), Ch.3, pp145-204. ISBN 978-0-8493-7314-5 (2007).

PROJECTS AND CONTRACTS

  • Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos (AVANSECAL-CM). Investigador Principal: Dra. Mª Luisa Marina Alegre (Universidad de Alcalá de Henares). 01/10/2014 – 31/09/2017. Programa de I+D en Tecnologías 2013, Dirección General de Universidades e Investigación, Consejería de Educación, Juventud y Deporte, Comunidad de Madrid.  S2013/ABI-3028.
  • Alimentos funcionales para una nutrición personalizada. Investigador Principal: Prof. Francisco A. Tomás Barberán (CEBAS-CSIC). 01/01/2014 – 31/12/2015. Redes de Excelencia, Secretaría de Estado de Investigación, Desarrollo e Innovación, MINECO.AGL2014-58205-REDC.
  • Café verde y yerba mate como fuente dietética de ácidos hidroxicinámicos. Estudios in vitro (cultivos celulares) e in vivo (humanos) de biodisponibilidad y bioactividad. Investigador principal: Laura Bravo Clemente. 01/10/2010 – 31/07/2015. MICINN. AGL2010-18269.
  • Efecto de los flavanoles de la dieta en la prevención del desarrollo de la diabetes tipo 2: estudio en cultivos celulares y animales de experimentación.  Investigador principal: Luis Goya Suárez. 01/01/2011 – 31/12/2014. MICINN. AGL2010-17579.
  • Guía para la sustanciación de declaraciones de salud en alimentos: funciones inmune, cognitiva y síndrome metabólico (INCOMES). Principal Investigator: Baltasar Ruiz-Roso Calvo de Mora and Vicente Lahera Juliá (Universidad Complutense de Madrid). 2013-2014.
  • Study of the bioavailability of hydroxytyrosil as functional ingredient in biscuits. ethers. Principal investigator: Laura Bravo Clemente. 2012-2013.
  • Evaluation of the antioxidant biological activity of a selenium-enriched fish. Principal investigator: Laura Bravo Clemente 2012.
  • Study of postprandial blood glucose and insulin after cocoa soluble products intake in a healthy population. Principal Investigator: Luis Goya. January-December, 2011. Intramural CSIC projects.
  • Green coffee and yerba mate as dietary sources of hydroxycinnamic acids. In vitro (cell culture) and in vivo (human) studies of bioavailability and bioactivity. Principal Investigator: Laura Bravo Clemente. 2010-2013. MICINN AGL2010-18269.
  • Preventive effects of dietary flavanols in type 2 diabetes: study in cell culture and experimental animals. Principal Investigator: Luis Goya. 2010-2013. MCINN: AGL2010-17579.
  • Vth International Congress on Polyphenols and Health (ICPH2011). Principal Investigator: Cristina de Andrés Lacueva (Barcelona University). 2010-2011. MICINN. Acción complementaria Modallidad A (Congress Organization).
  • Study of the potencial antiproliferative action of virgin olive oil polyphenols in human adenocarcinoma Caco-2 cells. Principal Investigator: Raquel Mateos Britz. January-December, 2010. Intramural Incorporation CSIC projects.
  • Utilization of byproducts from the olive industry. Synthesis and evaluation of new antioxidants with potential application to Parkinson disease. Principal Investigator: José Luis Espartero Sánchez. 2009-2012. Program of Excelence Projects from Consejería de Innovación, Ciencia y Empresa from Junta de Andalucía. AGR-5098.
  • Innovative analythical methodologies for quality and health control of foodstuffs (ANALYSIC-II). Principal Investigator: Mª José González Carlos (General Organic Chemistry Institute, CSIC). 2010-2013. Program of Activities of Research and Development of Research Groups of Comunidad de Madrid. P2009/AGR-1464.
  • Design and validation of active ingredients for developing functional food. Principal Investigator: Guillermo Reglero Rada (CIAL-CSIC). 2010-2013. Program of Activities of Research and Development of Research Groups of Comunidad de Madrid. P2009/AGR-1469.
  • Study of the potental antiinflammatory and anticarcinogenic effects of dietary quercetin in human hepatoma cells. Principal Investigaror: Sonia Ramos Rivero. 2008-2009. Intramural Incorporation CSIC projects.
  • Study of the cell bioavailability and antioxidant capacity of hydroxytyrosyl ethers. Principal Investigator: Laura Bravo Clemente. 2009-2012. Instituto Andaluz de Investigación y Formación Agraria y Pesquera (IFAPA). 110105090014.
  • New Ingredients and Functional foods to Improve Health. Principal Investigator: Francisco A. Tomás Barberán (CEBAS-CSIC). 2007-2013. Program Consolider-Ingenio 2010. CSD2007-00063.
  • In vitro and in vivo evaluation of the potential chemopreventive effect of dietary flavanols against colonic cancer. Principal Investigator: Laura Bravo Clemente. 2007-2010. CICYT. AGL2007-64042/ALI.
  • Preparation and evaluation of hydroxytyrosyl ethers as functional food ingredients. Principal Investigator: Arturo Cert Ventulá. Instituto de la Grasa – CSIC. 2007-2010. CICYT. AGL2007-64042/ALI.
  • Study of the functional properties of hydroxytyrosol esthers. Principal Investigator: Raquel Mateos Britz. National Institute for Agronomic Research -MICINN. 2007-2011. rta2007-00036-00-00.
  • Lipid peroxidation associated disorders: LPO. Principal Investigator: Neven Zarkovic (Ruder Boskovic Institute, Croacia). 2006-2010. European Union. COST Action-B35.
  • Study of functional properties of Maillard reaction products in thermically processed foodstuff. Principal Investigator: Francisco José Morales Navas. 2005-2008. CICYT. AGL2005-01735/ALI.
  • Study of the anticarcinogenic potential of dietary polyphenols and their metabolites in cell cultures and experimental animals. Principal Investigator: Sonia Ramos Rivero. 2004-2007.CICYT. AGL2004-00302/ALI.
  • Health implications of thermally processed foods. Principal Investigator: Vincenzo Fogliano (Universta’degli Studi di Napli Federico II, Italy). 2004-2009. European Union. COST Action -927.
  • Evaluation of the biological activity of fish rich in selinium. Principal Investigator: Laura Bravo Clemente. 2011-2012.
  • Study of the effect of two test samples on the antioxidant defences in a cell culture model. Principal Investigator: Luis Goya. 2010.
  • Study of the bioavailability of polyphenols and methylxanthines in soluble cocoa products and their postprandial effects on redox status in healthy volunteers. Principal Investigator: Laura Bravo Clemente. 2008-2011.
  • Study of the cardiovascular and intestinal habit effects of soluble cocoa products. Evaluation in healthy and at-health-risk volunteers. Principal Investigator: Laura Bravo Clemente.2008-2010.
  • Gastronomic Innovation. Scientific-Technological consultancy in the development of new food. Principal Investigator: Pilar Montero. 2007.
  • Evaluation of the potential of cocoa fiber in nutrition. Principal Investigator: Laura Bravo Clemente. 2005-2007.
  • Oxidative stress protection and anticarcinogenic potential of cocoa polyphenols. Principal Investigator: Luis Goya. 2004-2007.

PhD THESIS AND MASTERS

  • Mecanismos de acción de los flavanoles del cacao en las células hepáticas durante la resistencia a la insulina y la diabetes. Estudio en cultivos celulares y en animales de experimentación. Doctorando: Isabel Cordero Herrera. 2015. Director: Sonia Ramos Rivero. Universidad Complutense de Madrid, Facultad de Farmacia.
  • Metabolismo de flavonoides y ácidos hidroxicinámicos de la dieta. Estudios de transporte in vitro y de biodisponibilidad en humanos. Doctorando: Miren Gómez Juaristi. 2015. Directores: Laura Bravo Clemente y Raquel Mateos Briz. Universidad Complutense de Madrid. Facultad de Farmacia.
  • Cacao y café, alimentos ricos en fitoquímicos con propiedades beneficiosas para la salud. Estudios de biodisponibilidad en metilxantinas y efectos en salud en voluntarios sanos y con riesgo cardiovascular. Doctorando: Sara Martínez López. 2014. Directores: L Bravo, B Sarriá. Universidad: Complutense de Madrid, Facultad de Farmacia.
  • Nitrocatecoles derivados de hidroxitirosol. Síntesis, biodisponibilidad y evaluación de su capacidad antioxidante y neuroprotectora
    Doctorando: Elena Gallardo Morillo. 2014. Directores: José Antonio Gónzalez Correa, José Luis Espartero Sánchez y Raquel Mateos Briz. Universidad Málaga, Facultad de Farmacia.
  • PhD Thesis: Molecular mechanisms involved in the protective effect of dietary polyphenols against oxidative stress and inflammation. Study in colonic cells and experimental animals. PhD candidate: Ildefonso Rodríguez Ramiro. 2012. Directors: Drs. Luis Goya and Maria Ángeles Martín. University: Complutense of Madrid. School of Pharmacy.
  • PhD Thesis: Study of the bioavailability and biological activity of hydroxytyrosyl ethers in cell culture. PhD candidate: Gema Pereira Caro. 2011. PhD directors: Raquel Mateos Brtiz and Laura Bravo Clemente. University Complutense of Madrid. School of Pharmacy.
  • PhD Thesis: Chemopreventive effects of dietary polyphenols by modulation of apoptotic and survival/proliferation pathways in liver cells and experimental animals. PhD candidate: Ana Belén Granado-Serrano. 2009. PhD director: Sonia Ramos Rivero.
  • University Complutense of Madrid. School of Biological Sciences.
  • PhD Master: Metabolization of flavonoids in human cell lines. PhD candidate: Miren Gómez Juaristi. 2009. PhD director: Laura Bravo Clemente. University Complutense of Madrid. School of Pharmacy.
  • PhD Master: Study of dietary flavanol protection on redox status and insulin signalling in HepG2 cells submitted to high glucose concentrations. PhD candidate: Isabel Cordero. 2011. PhD: Director: Sonia Ramos Rivero. University Complutense of Madrid. School of Pharmacy.
  • PhD Master: Hydroxytyrosyl ethers. Synthesis and evaluation of the antioxidant capacity. PhD candidate: Gema Pereira Caro. 2007. PhD directors: Raquel Mateos Britz and Laura Bravo Clemente. Jaén University. School of Experimental Sciences.
  • PhD Master: Evaluation of the anticarcinogenic potential of quercetin polyphenol in cell studies. PhD candidate: Ana Belén Granado-Serrano. 2007. PhD director: Sonia Ramos Rivero. University Complutense of Madrid. School of Biological Sciences.